Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5520892

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant ENPP1 expressed in HEK293T cells using cGAMP as substrate at pH 7.5 measured after 3.5 hrs in presence of ATP by luminescence based AMP-glo assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of Pyrido[2,3-<i>d</i>]pyrimidin-7-one Derivatives as Highly Potent and Efficacious Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) Inhibitors for Cancer Treatment.
Sun Y, Chen M, Han Y, Li W, Ma X, Shi Z, Zhou Y, Xu L, Yu L, Wang Y, Yu J, Diao X, Meng L, Xu S.
J Med Chem (2024) 67 : 3986 -4006
PubMed 38387074 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 8.55 10.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5566114 1 10.05
CHEMBL5556000 1 9.80
CHEMBL5560511 1 9.52
CHEMBL5561670 1 9.32
CHEMBL5566818 1 9.25
CHEMBL5558246 1 9.18
CHEMBL5558533 1 9.14
CHEMBL5556506 1 9.10
CHEMBL5567749 1 9.03
CHEMBL5527873 1 8.85
CHEMBL5556761 1 8.75
CHEMBL5568520 1 8.67
CHEMBL5563471 1 8.62
CHEMBL5557235 1 8.39
CHEMBL5568392 1 8.39
CHEMBL5564529 1 8.21
CHEMBL5567338 1 8.21
CHEMBL5555461 1 8.07
CHEMBL5562733 1 7.90
CHEMBL1783609 1 7.78
CHEMBL5542628 1 7.72
CHEMBL5556763 1 7.47
CHEMBL5565761 1 6.94
CHEMBL5555308 1 6.80
CHEMBL5560400 1 -
CHEMBL5567993 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5566114 IC50 = 0.09 nM 10.05
CHEMBL5556000 IC50 = 0.16 nM 9.80
CHEMBL5560511 IC50 = 0.3 nM 9.52
CHEMBL5561670 IC50 = 0.48 nM 9.32
CHEMBL5566818 IC50 = 0.56 nM 9.25
CHEMBL5558246 IC50 = 0.66 nM 9.18
CHEMBL5558533 IC50 = 0.72 nM 9.14
CHEMBL5556506 IC50 = 0.8 nM 9.10
CHEMBL5567749 IC50 = 0.94 nM 9.03
CHEMBL5527873 IC50 = 1.41 nM 8.85
CHEMBL5556761 IC50 = 1.77 nM 8.75
CHEMBL5568520 IC50 = 2.14 nM 8.67
CHEMBL5563471 IC50 = 2.4 nM 8.62
CHEMBL5557235 IC50 = 4.03 nM 8.39
CHEMBL5568392 IC50 = 4.05 nM 8.39
CHEMBL5564529 IC50 = 6.19 nM 8.21
CHEMBL5567338 IC50 = 6.2 nM 8.21
CHEMBL5555461 IC50 = 8.46 nM 8.07
CHEMBL5562733 IC50 = 12.6 nM 7.90
CHEMBL1783609 IC50 = 16.7 nM 7.78
CHEMBL5542628 IC50 = 18.94 nM 7.72
CHEMBL5556763 IC50 = 34.04 nM 7.47
CHEMBL5565761 IC50 = 114.7 nM 6.94
CHEMBL5555308 IC50 = 157.1 nM 6.80
CHEMBL5560400 IC50 > 1000.0 nM -
CHEMBL5567993 IC50 > 1000.0 nM -