Assay Detail
Binding
CHEMBL5521333
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of interaction of human PD-1 expressed in Jurkat T cells co-transfected with NFAT/human PD-L1 expressed in CHO-K1 cells co-expressing aAPC preincubated with CHO-K1 cells followed by Jurkat cell addition and measured after 6 hrs by automated microplate spectrophotometer analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | CHO-K1 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Programmed cell death protein 1/Programmed cell death 1 ligand 1 (CHEMBL4523993) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Discovery of Novel PD-L1 Small-Molecular Inhibitors with Potent <i>In Vivo</i> Anti-tumor Immune Activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 6.63 | 6.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5562590 | — | — | 1 | 6.82 |
| CHEMBL5532575 | — | — | 1 | 6.80 |
| CHEMBL5563757 | — | — | 1 | 6.45 |
| CHEMBL5092744 | — | — | 1 | 6.45 |
| CHEMBL5559838 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5562590 | — | EC50 | = | 152.8 | nM | 6.82 |
| CHEMBL5532575 | — | EC50 | = | 158.4 | nM | 6.80 |
| CHEMBL5563757 | — | EC50 | = | 353.2 | nM | 6.45 |
| CHEMBL5092744 | — | EC50 | = | 356.4 | nM | 6.45 |
| CHEMBL5559838 | — | EC50 | - | — | - |