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Assay Detail

CHEMBL5521822

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal Met/6His tagged human recombinant matriptase catalytic domain (Gly596 to Val855 residues) using Boc-QAR-AMC as substrate preincubated for 30 mins followed by substrate addition and measured by fluorescence based analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Suppressor of tumorigenicity 14 protein (CHEMBL3018)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Mechanism-Based Macrocyclic Inhibitors of Serine Proteases.
Damalanka VC, Banas V, De Bona P, Kashipathy MM, Battaile K, Lovell S, Janetka JW.
J Med Chem (2024) 67 : 4833 -4854
PubMed 38477709 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.57 8.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5559738 1 8.54
CHEMBL5558547 1 8.47
CHEMBL5560415 1 8.24
CHEMBL5555448 1 8.01
CHEMBL5562350 1 7.85
CHEMBL5564865 1 7.77
CHEMBL5560759 1 7.50
CHEMBL5559988 1 7.41
CHEMBL5561725 1 7.27
CHEMBL5557087 1 7.24
CHEMBL5568169 1 7.05
CHEMBL5556134 1 7.04
CHEMBL5558228 1 6.85
CHEMBL5557551 1 6.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5559738 IC50 = 2.9 nM 8.54
CHEMBL5558547 IC50 = 3.4 nM 8.47
CHEMBL5560415 IC50 = 5.7 nM 8.24
CHEMBL5555448 IC50 = 9.8 nM 8.01
CHEMBL5562350 IC50 = 14.0 nM 7.85
CHEMBL5564865 IC50 = 17.0 nM 7.77
CHEMBL5560759 IC50 = 32.0 nM 7.50
CHEMBL5559988 IC50 = 39.0 nM 7.41
CHEMBL5561725 IC50 = 54.0 nM 7.27
CHEMBL5557087 IC50 = 57.0 nM 7.24
CHEMBL5568169 IC50 = 90.0 nM 7.05
CHEMBL5556134 IC50 = 91.0 nM 7.04
CHEMBL5558228 IC50 = 140.0 nM 6.85
CHEMBL5557551 IC50 = 180.0 nM 6.75