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Assay Detail

CHEMBL5521824

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant thrombin (unknown origin) using D-Phe-Pip-Arg-pNA as chromogenic substrate preincubated for 30 mins followed by substrate addition by absorbance based analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prothrombin (CHEMBL204)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Mechanism-Based Macrocyclic Inhibitors of Serine Proteases.
Damalanka VC, Banas V, De Bona P, Kashipathy MM, Battaile K, Lovell S, Janetka JW.
J Med Chem (2024) 67 : 4833 -4854
PubMed 38477709 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.61 6.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5558547 1 6.91
CHEMBL5568169 1 6.02
CHEMBL5559738 1 5.96
CHEMBL5555448 1 5.91
CHEMBL5560415 1 5.87
CHEMBL5560759 1 5.58
CHEMBL5561725 1 5.50
CHEMBL5559988 1 5.37
CHEMBL5564865 1 5.06
CHEMBL5562350 1 5.05
CHEMBL5557087 1 5.02
CHEMBL5556134 1 5.02
CHEMBL5557551 1 -
CHEMBL5558228 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5558547 IC50 = 122.0 nM 6.91
CHEMBL5568169 IC50 = 955.0 nM 6.02
CHEMBL5559738 IC50 = 1110.0 nM 5.96
CHEMBL5555448 IC50 = 1240.0 nM 5.91
CHEMBL5560415 IC50 = 1360.0 nM 5.87
CHEMBL5560759 IC50 = 2646.0 nM 5.58
CHEMBL5561725 IC50 = 3180.0 nM 5.50
CHEMBL5559988 IC50 = 4290.0 nM 5.37
CHEMBL5564865 IC50 = 8700.0 nM 5.06
CHEMBL5562350 IC50 = 8810.0 nM 5.05
CHEMBL5557087 IC50 = 9530.0 nM 5.02
CHEMBL5556134 IC50 = 9540.0 nM 5.02
CHEMBL5557551 IC50 - -
CHEMBL5558228 IC50 - -