Assay Detail
Binding
CHEMBL5523023
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of NIK (unknown origin) expressed in COS-7 cells co-expressing NF-KappaB luciferase reporter assessed as suppression of luciferase reporter measured after 24 by beta-Gal activity assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | COS-7 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Mitogen-activated protein kinase kinase kinase 14 (CHEMBL5888) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Design, Synthesis, and Biological Evaluation of a Novel NIK Inhibitor with Anti-Inflammatory and Hepatoprotective Effects for Sepsis Treatment.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 5.76 | 6.04 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3187788 | — | — | 1 | 6.04 |
| CHEMBL5562073 | — | — | 1 | 5.92 |
| CHEMBL5542106 | — | — | 1 | 5.88 |
| CHEMBL5556543 | — | — | 1 | 5.85 |
| CHEMBL5567931 | — | — | 1 | 5.82 |
| CHEMBL5564890 | — | — | 1 | 5.66 |
| CHEMBL5542501 | — | — | 1 | 5.65 |
| CHEMBL5542810 | — | — | 1 | 5.61 |
| CHEMBL5523606 | — | — | 1 | 5.45 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3187788 | — | IC50 | = | 910.0 | nM | 6.04 |
| CHEMBL5562073 | — | IC50 | = | 1190.0 | nM | 5.92 |
| CHEMBL5542106 | — | IC50 | = | 1330.0 | nM | 5.88 |
| CHEMBL5556543 | — | IC50 | = | 1410.0 | nM | 5.85 |
| CHEMBL5567931 | — | IC50 | = | 1510.0 | nM | 5.82 |
| CHEMBL5564890 | — | IC50 | = | 2170.0 | nM | 5.66 |
| CHEMBL5542501 | — | IC50 | = | 2230.0 | nM | 5.65 |
| CHEMBL5542810 | — | IC50 | = | 2430.0 | nM | 5.61 |
| CHEMBL5523606 | — | IC50 | = | 3560.0 | nM | 5.45 |