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Assay Detail

CHEMBL5524258

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal His-tagged recombinant human TET2 catalytic domain LCIdel mutant (D1129 to G1936 residues with Y1481 to N1843 replaced by 3 x GGGGS linker) expressed in Escherichia coli BL21 (DE3) using 5-methylcytosine as substrate preincubated for 10 mins followed by substrate and DNA-cofactor addition measured after 12 mins by SPE-MS analysis
18
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Methylcytosine dioxygenase TET2 (CHEMBL4523344)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective targeting of human TET1 by cyclic peptide inhibitors: Insights from biochemical profiling.
Šimelis K, Saraç H, Salah E, Nishio K, McAllister TE, Corner TP, Tumber A, Belle R, Schofield CJ, Suga H, Kawamura A.
Bioorg Med Chem (2024) 99 : 117597 -117597
PubMed 38262305 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.44 6.26
pIC50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1230640 1 6.26
CHEMBL5560200 1 5.66
CHEMBL5557957 1 5.61
CHEMBL5549950 1 5.60
CHEMBL5561920 1 5.55
CHEMBL5561497 1 5.54
CHEMBL5532540 1 5.52
CHEMBL5549844 1 5.51
CHEMBL5563232 1 5.50
CHEMBL5557587 1 5.47
CHEMBL5564980 1 5.44
CHEMBL5562105 1 5.40
CHEMBL5557372 1 5.27
CHEMBL5557793 1 5.19
CHEMBL5561894 1 4.85
CHEMBL5564944 1 4.62
CHEMBL5542589 1 -
CHEMBL5567655 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1230640 IC50 = 549.54 nM 6.26
CHEMBL5560200 IC50 = 2187.76 nM 5.66
CHEMBL5557957 IC50 = 2454.71 nM 5.61
CHEMBL5549950 IC50 = 2511.89 nM 5.60
CHEMBL5561920 IC50 = 2818.38 nM 5.55
CHEMBL5561497 IC50 = 2884.03 nM 5.54
CHEMBL5532540 IC50 = 3019.95 nM 5.52
CHEMBL5549844 IC50 = 3090.3 nM 5.51
CHEMBL5563232 IC50 = 3162.28 nM 5.50
CHEMBL5557587 IC50 = 3388.44 nM 5.47
CHEMBL5564980 IC50 = 3630.78 nM 5.44
CHEMBL5562105 IC50 = 3981.07 nM 5.40
CHEMBL5557372 IC50 = 5370.32 nM 5.27
CHEMBL5557793 IC50 = 6456.54 nM 5.19
CHEMBL5561894 IC50 = 14125.38 nM 4.85
CHEMBL5564944 IC50 = 23988.33 nM 4.62
CHEMBL5542589 pIC50 - -
CHEMBL5567655 IC50 ~ 10000.0 nM -