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Assay Detail

CHEMBL5526174

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 125I-rat amylin from rat AMY3R complex of CTR/RAMP3 expressed in Baby hamster kidney cell membrane incubated for 120 mins by SPA method
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Kidney
Cell Type Baby hamster kidney cell
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Amylin receptor AMY3; CALCR/RAMP3 (CHEMBL2109235)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

NN1213 - A Potent, Long-Acting, and Selective Analog of Human Amylin.
Dahl K, Raun K, Hansen JL, Poulsen C, de la Cour CD, Clausen TR, Hansen AMK, John LM, Plesner A, Sun G, Schlein M, Skyggebjerg RB, Kruse T.
J Med Chem (2024) 67 : 11688 -11700
PubMed 38960379 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 9.22 10.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3989767 CALCITONIN SALMON 4.0 1 10.55
CHEMBL2103758 PRAMLINTIDE 4.0 1 9.91
CHEMBL5567107 1 9.75
CHEMBL5554990 1 9.69
CHEMBL5567446 1 9.66
CHEMBL5567377 1 9.54
CHEMBL5566450 1 9.43
CHEMBL5556440 1 9.35
CHEMBL5561117 1 9.04
CHEMBL5568414 1 9.00
CHEMBL5568431 1 8.93
CHEMBL5560524 1 8.91
CHEMBL5560328 1 8.89
CHEMBL5568471 1 8.89
CHEMBL5566453 1 8.77
CHEMBL5568545 1 8.75
CHEMBL5568143 1 8.52
CHEMBL5565993 1 8.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3989767 CALCITONIN SALMON IC50 = 0.028 nM 10.55
CHEMBL2103758 PRAMLINTIDE IC50 = 0.122 nM 9.91
CHEMBL5567107 IC50 = 0.176 nM 9.75
CHEMBL5554990 IC50 = 0.203 nM 9.69
CHEMBL5567446 IC50 = 0.217 nM 9.66
CHEMBL5567377 IC50 = 0.29 nM 9.54
CHEMBL5566450 IC50 = 0.372 nM 9.43
CHEMBL5556440 IC50 = 0.449 nM 9.35
CHEMBL5561117 IC50 = 0.92 nM 9.04
CHEMBL5568414 IC50 = 1.002 nM 9.00
CHEMBL5568431 IC50 = 1.171 nM 8.93
CHEMBL5560524 IC50 = 1.24 nM 8.91
CHEMBL5560328 IC50 = 1.293 nM 8.89
CHEMBL5568471 IC50 = 1.3 nM 8.89
CHEMBL5566453 IC50 = 1.715 nM 8.77
CHEMBL5568545 IC50 = 1.768 nM 8.75
CHEMBL5568143 IC50 = 3.006 nM 8.52
CHEMBL5565993 IC50 = 4.69 nM 8.33