Assay Detail
Binding
CHEMBL5529508
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Antagonist activity at human NOD2 expressed in HEK-Blue cells coexpressing NF-kappaB-inducible SEAP reporter gene assessed as inhibition of MDP-induced NF-kappaB transcriptional activity by measuring decrease in SEAP activity preincubated for 1 hr followed by MDP addition and measured after 18 hrs by spectrophotometric analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK-Blue hNOD2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Nucleotide-binding oligomerization domain-containing protein 2 (CHEMBL1293266) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of 2,3-Diaminoindole Derivatives as a Novel Class of NOD Antagonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 4.50 | 4.79 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5566720 | — | — | 1 | 4.79 |
| CHEMBL5595306 | — | — | 1 | 4.68 |
| CHEMBL5591686 | — | — | 1 | 4.38 |
| CHEMBL5594651 | — | — | 1 | 4.37 |
| CHEMBL1412853 | — | — | 1 | 4.26 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5566720 | — | IC50 | = | 16100.0 | nM | 4.79 |
| CHEMBL5595306 | — | IC50 | = | 20800.0 | nM | 4.68 |
| CHEMBL5591686 | — | IC50 | = | 41900.0 | nM | 4.38 |
| CHEMBL5594651 | — | IC50 | = | 42600.0 | nM | 4.37 |
| CHEMBL1412853 | — | IC50 | = | 54900.0 | nM | 4.26 |