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Assay Detail

CHEMBL5531642

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Staphylococcus aureus DHFR expressed in Escherichia coli BL21 (DE3) using DHF as substrate preincubated for 10 mins followed by substrate addition measured for 10 mins in presence of NADPH by spectrophotometry
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL1681620)
Type SINGLE PROTEIN
Organism Staphylococcus aureus

Publication

Design, Synthesis, and Biological Evaluation of 5-(5-Iodo-2-isopropyl-4-methoxyphenoxy)pyrimidine-2,4-diamine (AF-353) Derivatives as Novel DHFR Inhibitors against <i>Staphylococcus aureus</i>.
Huang Z, Gou X, Hang X, Shi T, Yang J, Liu Y, He X, Li J, Quan K, Bi H, Luo Y.
J Med Chem (2024) 67 : 4757 -4781
PubMed 38466654 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 7.62 9.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5563206 1 9.01
CHEMBL5562750 1 8.74
CHEMBL5569444 1 8.35
CHEMBL5575458 1 8.11
CHEMBL5569040 1 8.08
CHEMBL5572153 1 8.08
CHEMBL5562500 1 7.99
CHEMBL5564319 1 7.81
CHEMBL5566054 1 7.80
CHEMBL5550068 1 7.72
CHEMBL5574252 1 7.62
CHEMBL502618 1 7.61
CHEMBL22 TRIMETHOPRIM 4.0 1 7.58
CHEMBL5575103 1 7.55
CHEMBL526307 1 7.48
CHEMBL5575570 1 7.46
CHEMBL5589963 1 7.43
CHEMBL5572967 1 7.42
CHEMBL5563617 1 7.41
CHEMBL5565406 1 7.38
CHEMBL5590270 1 7.35
CHEMBL5564050 1 7.22
CHEMBL5556334 1 7.18
CHEMBL5569247 1 7.16
CHEMBL5565112 1 6.85
CHEMBL5569505 1 6.83
CHEMBL5572687 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5563206 IC50 = 0.97 nM 9.01
CHEMBL5562750 IC50 = 1.81 nM 8.74
CHEMBL5569444 IC50 = 4.44 nM 8.35
CHEMBL5575458 IC50 = 7.69 nM 8.11
CHEMBL5569040 IC50 = 8.29 nM 8.08
CHEMBL5572153 IC50 = 8.36 nM 8.08
CHEMBL5562500 IC50 = 10.16 nM 7.99
CHEMBL5564319 IC50 = 15.45 nM 7.81
CHEMBL5566054 IC50 = 15.9 nM 7.80
CHEMBL5550068 IC50 = 19.06 nM 7.72
CHEMBL5574252 IC50 = 24.2 nM 7.62
CHEMBL502618 IC50 = 24.71 nM 7.61
CHEMBL22 TRIMETHOPRIM IC50 = 26.46 nM 7.58
CHEMBL5575103 IC50 = 27.84 nM 7.55
CHEMBL526307 IC50 = 32.87 nM 7.48
CHEMBL5575570 IC50 = 34.84 nM 7.46
CHEMBL5589963 IC50 = 36.7 nM 7.43
CHEMBL5572967 IC50 = 37.89 nM 7.42
CHEMBL5563617 IC50 = 38.66 nM 7.41
CHEMBL5565406 IC50 = 41.8 nM 7.38
CHEMBL5590270 IC50 = 45.03 nM 7.35
CHEMBL5564050 IC50 = 60.23 nM 7.22
CHEMBL5556334 IC50 = 66.62 nM 7.18
CHEMBL5569247 IC50 = 69.52 nM 7.16
CHEMBL5565112 IC50 = 142.5 nM 6.85
CHEMBL5569505 IC50 = 148.5 nM 6.83
CHEMBL5572687 IC50 = 238.9 nM 6.62