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Assay Detail

CHEMBL5531697

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human URAT1 expressed in HEK293T cells using 14C- uric acid assessed as uric acid absorption by measuring intracellular liquid radiation value by liquid scintillation counting analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Solute carrier family 22 member 12 (CHEMBL6120)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Novel Thienopyrimidine Compound as a Urate Transporter 1 and Glucose Transporter 9 Dual Inhibitor with Improved Efficacy and Favorable Druggability.
Shi X, Zhao T, Wang S, Xu S, Liao H, Gao S, Gao Z, Zhang J, Qi D, Zhang Z, Zheng F, Wang Y, Wang Z, Yang M, Yang Q, Yi F, Pang J, Liu X, Zhan P.
J Med Chem (2024) 67 : 5032 -5052
PubMed 38482820 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.44 5.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5590320 1 5.70
CHEMBL5542414 1 5.60
CHEMBL5579679 1 5.56
CHEMBL5570059 1 5.43
CHEMBL5565332 1 5.40
CHEMBL5555561 1 5.36
CHEMBL5573620 1 5.33
CHEMBL5571882 1 5.31
CHEMBL2105720 LESINURAD 4.0 1 5.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5590320 IC50 = 2010.0 nM 5.70
CHEMBL5542414 IC50 = 2510.0 nM 5.60
CHEMBL5579679 IC50 = 2750.0 nM 5.56
CHEMBL5570059 IC50 = 3750.0 nM 5.43
CHEMBL5565332 IC50 = 3960.0 nM 5.40
CHEMBL5555561 IC50 = 4350.0 nM 5.36
CHEMBL5573620 IC50 = 4730.0 nM 5.33
CHEMBL5571882 IC50 = 4860.0 nM 5.31
CHEMBL2105720 LESINURAD IC50 = 5540.0 nM 5.26