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Assay Detail

CHEMBL5534724

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to EGFR L858R mutant (unknown origin) assessed as apparent inhibition constant in presence of ATP by TR-FRET assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pitfalls and Considerations in Determining the Potency and Mutant Selectivity of Covalent Epidermal Growth Factor Receptor Inhibitors.
Hoyt KW, Urul DA, Ogboo BC, Wittlinger F, Laufer SA, Schaefer EM, May EW, Heppner DE.
J Med Chem (2024) 67 : 2 -16
PubMed 38134304 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 8.53 8.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4098072 1 8.90
CHEMBL3353410 OSIMERTINIB 4.0 1 8.35
CHEMBL4558324 LAZERTINIB 4.0 1 8.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4098072 Ki = 1.27 nM 8.90
CHEMBL3353410 OSIMERTINIB Ki = 4.47 nM 8.35
CHEMBL4558324 LAZERTINIB Ki = 4.68 nM 8.33