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Assay Detail

CHEMBL5535654

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to HCV IRES subdomain IIa RNA assessed as reduction in FRET signal by FRET assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepacivirus hominis
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Target

Internal ribosome entry site (IRES) (CHEMBL612412)
Type NUCLEIC-ACID
Organism Hepacivirus hominis

Publication

Structure-based virtual screening of unbiased and RNA-focused libraries to identify new ligands for the HCV IRES model system.
Kallert E, Almena Rodriguez L, Husmann JÅ, Blatt K, Kersten C.
RSC Med Chem (2024) 15 : 1527 -1538
PubMed 38784459 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 4.42 5.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL223608 1 5.31
CHEMBL5564851 1 4.21
CHEMBL427101 1 4.11
CHEMBL5570170 1 4.06
CHEMBL5575339 1 -
CHEMBL581893 1 -
CHEMBL5592337 1 -
CHEMBL5563196 1 -
CHEMBL5583536 1 -
CHEMBL5571553 1 -
CHEMBL5575784 1 -
CHEMBL945 AMILORIDE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL223608 EC50 = 4900.0 nM 5.31
CHEMBL5564851 EC50 = 61000.0 nM 4.21
CHEMBL427101 EC50 = 78000.0 nM 4.11
CHEMBL5570170 EC50 = 88000.0 nM 4.06
CHEMBL5575339 EC50 = 413000.0 nM -
CHEMBL581893 EC50 = 120000.0 nM -
CHEMBL5592337 EC50 = 217000.0 nM -
CHEMBL5563196 EC50 >= 500000.0 nM -
CHEMBL5583536 EC50 >= 500000.0 nM -
CHEMBL5571553 EC50 >= 300000.0 nM -
CHEMBL5575784 EC50 >= 1000000.0 nM -
CHEMBL945 AMILORIDE EC50 >= 500000.0 nM -