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Assay Detail

CHEMBL5536361

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Cathepsin S (unknown origin) expressed in Escherichia coli using Z-Val-Val-Arg-AMC as substrate by fluorescence based assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cathepsin S (CHEMBL2954)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural Modifications of Covalent Cathepsin S Inhibitors: Impact on Affinity, Selectivity, and Permeability.
Meta M, Zimmer C, Fuchs N, Zecher MJ, Lahu A, Schirmeister T.
ACS Med Chem Lett (2024) 15 : 837 -844
PubMed 38894911 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 26 7.91 10.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5573840 1 10.41
CHEMBL5567047 1 9.30
CHEMBL249915 1 9.00
CHEMBL347111 1 8.92
CHEMBL2207560 1 8.82
CHEMBL5575199 1 8.66
CHEMBL5566228 1 8.62
CHEMBL5593543 1 8.52
CHEMBL5591918 1 8.44
CHEMBL153239 1 8.24
CHEMBL5573367 1 8.11
CHEMBL5571616 1 7.96
CHEMBL356155 1 7.96
CHEMBL5574900 1 7.89
CHEMBL5572314 1 7.89
CHEMBL5549958 1 7.89
CHEMBL5574337 1 7.70
CHEMBL5572340 1 7.60
CHEMBL5567080 1 7.21
CHEMBL5589673 1 7.17
CHEMBL5595801 1 6.93
CHEMBL5592727 1 6.84
CHEMBL5594049 1 6.78
CHEMBL5570914 1 6.48
CHEMBL5564109 1 6.42
CHEMBL5590540 1 6.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5573840 Ki = 0.039 nM 10.41
CHEMBL5567047 Ki = 0.5 nM 9.30
CHEMBL249915 Ki = 1.0 nM 9.00
CHEMBL347111 Ki = 1.2 nM 8.92
CHEMBL2207560 Ki = 1.5 nM 8.82
CHEMBL5575199 Ki = 2.2 nM 8.66
CHEMBL5566228 Ki = 2.4 nM 8.62
CHEMBL5593543 Ki = 3.0 nM 8.52
CHEMBL5591918 Ki = 3.6 nM 8.44
CHEMBL153239 Ki = 5.7 nM 8.24
CHEMBL5573367 Ki = 7.8 nM 8.11
CHEMBL5571616 Ki = 11.0 nM 7.96
CHEMBL356155 Ki = 11.0 nM 7.96
CHEMBL5574900 Ki = 13.0 nM 7.89
CHEMBL5572314 Ki = 13.0 nM 7.89
CHEMBL5549958 Ki = 13.0 nM 7.89
CHEMBL5574337 Ki = 20.0 nM 7.70
CHEMBL5572340 Ki = 25.0 nM 7.60
CHEMBL5567080 Ki = 61.0 nM 7.21
CHEMBL5589673 Ki = 67.0 nM 7.17
CHEMBL5595801 Ki = 117.0 nM 6.93
CHEMBL5592727 Ki = 146.0 nM 6.84
CHEMBL5594049 Ki = 165.0 nM 6.78
CHEMBL5570914 Ki = 331.0 nM 6.48
CHEMBL5564109 Ki = 382.0 nM 6.42
CHEMBL5590540 Ki = 989.0 nM 6.00