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Assay Detail

CHEMBL5536364

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to recombinant Cathepsin S (unknown origin) expressed in Escherichia coli using Z-Val-Val-Arg-AMC as substrate assessed as inhibition constant for slowly reversible binding by fluorescence based assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cathepsin S (CHEMBL2954)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural Modifications of Covalent Cathepsin S Inhibitors: Impact on Affinity, Selectivity, and Permeability.
Meta M, Zimmer C, Fuchs N, Zecher MJ, Lahu A, Schirmeister T.
ACS Med Chem Lett (2024) 15 : 837 -844
PubMed 38894911 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 9.09 10.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5573840 1 10.80
CHEMBL5570914 1 7.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5573840 Ki = 0.016 nM 10.80
CHEMBL5570914 Ki = 43.0 nM 7.37