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Assay Detail

CHEMBL5537720

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant HDAC6 using Boc-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 90 mins by fluorescence based microplate reader analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

5-(Trifluoromethyl)-1,2,4-oxadiazole (TFMO)-based highly selective class IIa HDAC inhibitors exhibit synergistic anticancer activity in combination with bortezomib.
Asfaha Y, Bollmann LM, Skerhut AJ, Fischer F, Horstick N, Roth D, Wecker M, Mammen C, Smits SHJ, Fluegen G, Kassack MU, Kurz T.
Eur J Med Chem (2024) 263 : 115907 -115907
PubMed 37979441 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 4.95 5.69

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5563683 1 5.69
CHEMBL5571522 1 5.25
CHEMBL3692688 1 5.24
CHEMBL5571546 1 4.94
CHEMBL5570082 1 4.77
CHEMBL5575926 1 4.74
CHEMBL5571764 1 4.71
CHEMBL3692573 1 4.26
CHEMBL5572569 1 -
CHEMBL5571685 1 -
CHEMBL5579748 1 -
CHEMBL5572470 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5563683 IC50 = 2030.0 nM 5.69
CHEMBL5571522 IC50 = 5610.0 nM 5.25
CHEMBL3692688 IC50 = 5790.0 nM 5.24
CHEMBL5571546 IC50 = 11400.0 nM 4.94
CHEMBL5570082 IC50 = 16900.0 nM 4.77
CHEMBL5575926 IC50 = 18100.0 nM 4.74
CHEMBL5571764 IC50 = 19600.0 nM 4.71
CHEMBL3692573 IC50 = 55400.0 nM 4.26
CHEMBL5572569 IC50 > 100000.0 nM -
CHEMBL5571685 IC50 > 100000.0 nM -
CHEMBL5579748 IC50 > 100000.0 nM -
CHEMBL5572470 IC50 > 100000.0 nM -