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Assay Detail

CHEMBL5539985

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PHD2 (unknown origin) preincubated for 30 mins followed by substrate addition and measured after 30 mins by HTS plate reader analysis
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Egl nine homolog 1 (CHEMBL5697)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel and Potent Prolyl Hydroxylase Domain-Containing Protein (PHD) Inhibitors for The Treatment of Anemia.
Xu J, Ding X, Fu Y, Meng Q, Wang L, Zhang M, Xu C, Chen S, Aliper A, Ren F, Zhavoronkov A, Ding X.
J Med Chem (2024) 67 : 1393 -1405
PubMed 38189253 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 8.09 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5572635 1 9.00
CHEMBL5590779 1 9.00
CHEMBL5564592 1 8.70
CHEMBL5574218 1 8.70
CHEMBL5565890 1 8.70
CHEMBL5563061 1 8.60
CHEMBL5572587 1 8.52
CHEMBL5575448 1 8.40
CHEMBL5572628 1 8.40
CHEMBL5594033 1 8.40
CHEMBL5567289 1 8.05
CHEMBL2338329 ROXADUSTAT 4.0 1 7.85
CHEMBL5571964 1 7.72
CHEMBL5562929 1 7.43
CHEMBL5583677 1 7.06
CHEMBL5594247 1 6.54
CHEMBL5572863 1 6.53
CHEMBL5572357 1 -
CHEMBL5570245 1 -
CHEMBL5572667 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5572635 IC50 = 1.0 nM 9.00
CHEMBL5590779 IC50 = 1.0 nM 9.00
CHEMBL5564592 IC50 = 2.0 nM 8.70
CHEMBL5574218 IC50 = 2.0 nM 8.70
CHEMBL5565890 IC50 = 2.0 nM 8.70
CHEMBL5563061 IC50 = 2.5 nM 8.60
CHEMBL5572587 IC50 = 3.0 nM 8.52
CHEMBL5575448 IC50 = 4.0 nM 8.40
CHEMBL5572628 IC50 = 4.0 nM 8.40
CHEMBL5594033 IC50 = 4.0 nM 8.40
CHEMBL5567289 IC50 = 9.0 nM 8.05
CHEMBL2338329 ROXADUSTAT IC50 = 14.0 nM 7.85
CHEMBL5571964 IC50 = 19.0 nM 7.72
CHEMBL5562929 IC50 = 37.0 nM 7.43
CHEMBL5583677 IC50 = 87.0 nM 7.06
CHEMBL5594247 IC50 = 291.0 nM 6.54
CHEMBL5572863 IC50 = 297.0 nM 6.53
CHEMBL5572357 IC50 > 20000.0 nM -
CHEMBL5570245 IC50 > 20000.0 nM -
CHEMBL5572667 IC50 > 2000.0 nM -