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Assay Detail

CHEMBL5540230

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PKCalpha (unknown origin) incubated for 15 mins in presence of 33P-ATP by Topcount scintillation counting method
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein kinase C alpha type (CHEMBL299)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Darovasertib (NVP-LXS196), a Pan-PKC Inhibitor for the Treatment of Metastatic Uveal Melanoma.
Visser M, Papillon JPN, Luzzio M, LaMarche MJ, Fan J, Michael W, Wang D, Zhang A, Straub C, Mathieu S, Kato M, Palermo M, Chen C, Ramsey T, Joud C, Barrett R, Vattay A, Guo R, Bric A, Chung F, Liang G, Romanowski MJ, Lam J, Thohan S, Atassi F, Wylie A, Cooke VG.
J Med Chem (2024) 67 : 1447 -1459
PubMed 38198520 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 8.77 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3946659 1 9.70
CHEMBL3957149 1 9.70
CHEMBL5592487 1 9.70
CHEMBL3965722 1 9.52
CHEMBL3950697 1 9.52
CHEMBL5566264 1 9.40
CHEMBL565612 SOTRASTAURIN 2.0 1 9.10
CHEMBL3924825 1 9.08
CHEMBL3890986 1 8.70
CHEMBL3982723 DAROVASERTIB 2.0 1 8.52
CHEMBL3915489 1 8.52
CHEMBL5564760 1 8.00
CHEMBL5565713 1 7.96
CHEMBL5571382 1 7.37
CHEMBL5591535 1 6.75
CHEMBL3943147 1 -
CHEMBL3966643 1 -
CHEMBL3982497 1 -
CHEMBL3957715 1 -
CHEMBL3966595 1 -
CHEMBL5573642 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3946659 IC50 = 0.2 nM 9.70
CHEMBL3957149 IC50 = 0.2 nM 9.70
CHEMBL5592487 IC50 = 0.2 nM 9.70
CHEMBL3965722 IC50 = 0.3 nM 9.52
CHEMBL3950697 IC50 = 0.3 nM 9.52
CHEMBL5566264 IC50 = 0.4 nM 9.40
CHEMBL565612 SOTRASTAURIN IC50 = 0.8 nM 9.10
CHEMBL3924825 IC50 = 0.84 nM 9.08
CHEMBL3890986 IC50 = 2.0 nM 8.70
CHEMBL3982723 DAROVASERTIB IC50 = 3.0 nM 8.52
CHEMBL3915489 IC50 = 3.0 nM 8.52
CHEMBL5564760 IC50 = 10.0 nM 8.00
CHEMBL5565713 IC50 = 11.0 nM 7.96
CHEMBL5571382 IC50 = 43.0 nM 7.37
CHEMBL5591535 IC50 = 180.0 nM 6.75
CHEMBL3943147 IC50 < 0.1 nM -
CHEMBL3966643 IC50 < 0.1 nM -
CHEMBL3982497 IC50 < 0.1 nM -
CHEMBL3957715 IC50 < 0.1 nM -
CHEMBL3966595 IC50 < 0.13 nM -
CHEMBL5573642 IC50 < 0.1 nM -