Assay Detail
Functional
CHEMBL5547468
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at human recombinant adrenergic alpha-2A receptor expressed in CHO-K1 cells co-transfected with 22F-Glosensor assessed as inhibition of cAMP production measured after 30 mins incubation by Microbeta2Micro-plate counter analysis
4
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | CHO-K1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and Structure-Activity Relationships of 2,5-Dimethoxy-4-Substituted Phenethylamines and the Discovery of CYB210010: A Potent, Orally Bioavailable and Long-Acting Serotonin 5-HT<sub>2</sub> Receptor Agonist.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 8.21 | 9.54 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL134 | CLONIDINE | 4.0 | 2 | 9.54 |
| CHEMBL5566821 | — | — | 2 | 6.90 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL134 | CLONIDINE | EC50 | = | 0.29 | nM | 9.54 |
| CHEMBL134 | CLONIDINE | EC50 | = | 0.3162 | nM | 9.50 |
| CHEMBL5566821 | — | EC50 | = | 126.0 | nM | 6.90 |
| CHEMBL5566821 | — | EC50 | = | 125.89 | nM | 6.90 |