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Assay Detail

CHEMBL5550833

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS-CoV-2 main protease BetaCoV/Wuhan/WIV04/2019 expressed in Escherichia coli BL21(DE3) cells using FRET as substrate incubated for 1 hr
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Exploiting high-energy hydration sites for the discovery of potent peptide aldehyde inhibitors of the SARS-CoV-2 main protease with cellular antiviral activity.
Carney DW, Leffler AE, Bell JA, Chandrasinghe AS, Cheng C, Chang E, Dornford A, Dougan DR, Frye LL, Grimes ME, Knehans T, Knight JL, Komandla M, Lane W, Li H, Newman SR, Phimister K, Saikatendu KS, Silverstein H, Vafaei S.
Bioorg Med Chem (2024) 103 : 117577 -117577
PubMed 38518735 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.06 6.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL318779 1 6.06
CHEMBL218394 BOCEPREVIR 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL318779 IC50 = 870.0 nM 6.06
CHEMBL218394 BOCEPREVIR IC50 = 4.1 nM -