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Assay Detail

CHEMBL5553735

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Binding
Binding affinity to recombinant his tagged GAK (unknown origin) assessed as dissociation constant by surface plasmon resonance method
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cyclin-G-associated kinase (CHEMBL4355)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Back-Pocket Optimization of 2-Aminopyrimidine-Based Macrocycles Leads to Potent EPHA2/GAK Kinase Inhibitors.
Gerninghaus J, Zhubi R, Krämer A, Karim M, Tran DHN, Joerger AC, Schreiber C, Berger LM, Berger BT, Ehret TAL, Elson L, Lenz C, Saxena K, Müller S, Einav S, Knapp S, Hanke T.
J Med Chem (2024) 67 : 12534 -12552
PubMed 39028937 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 6 7.81 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5566270 1 8.30
CHEMBL5574808 1 8.21
CHEMBL5565547 1 8.13
CHEMBL5568747 1 7.85
CHEMBL5574265 1 7.44
CHEMBL1235529 1 6.95

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5566270 Kd = 5.0 nM 8.30
CHEMBL5574808 Kd = 6.2 nM 8.21
CHEMBL5565547 Kd = 7.4 nM 8.13
CHEMBL5568747 Kd = 14.1 nM 7.85
CHEMBL5574265 Kd = 36.6 nM 7.44
CHEMBL1235529 Kd = 112.4 nM 6.95