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Assay Detail

CHEMBL5554615

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human P2X2/3R expressed in HEK293 cells incubated for 18 hrs by Fluo-4 dye based microplate reader assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

P2X2/P2X3 heterotrimeric receptor (CHEMBL3831281)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of Triazolopyrimidine Derivatives as Selective P2X3 Receptor Antagonists Binding to an Unprecedented Allosteric Site as Evidenced by Cryo-Electron Microscopy.
Kim GR, Kim S, Kim YO, Han X, Nagel J, Kim J, Song DI, Müller CE, Yoon MH, Jin MS, Kim YC.
J Med Chem (2024) 67 : 14443 -14465
PubMed 39102524 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.92 7.71

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL526307 1 7.71
CHEMBL5573155 1 6.05
CHEMBL5574288 1 5.93
CHEMBL5594973 1 5.93
CHEMBL5574915 1 5.89
CHEMBL5572710 1 5.84
CHEMBL5590914 1 5.69
CHEMBL5574190 1 5.65
CHEMBL5207245 1 5.30
CHEMBL5564655 1 5.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL526307 IC50 = 19.6 nM 7.71
CHEMBL5573155 IC50 = 881.0 nM 6.05
CHEMBL5574288 IC50 = 1186.0 nM 5.93
CHEMBL5594973 IC50 = 1180.0 nM 5.93
CHEMBL5574915 IC50 = 1290.0 nM 5.89
CHEMBL5572710 IC50 = 1451.0 nM 5.84
CHEMBL5590914 IC50 = 2028.0 nM 5.69
CHEMBL5574190 IC50 = 2254.0 nM 5.65
CHEMBL5207245 IC50 = 5060.0 nM 5.30
CHEMBL5564655 IC50 = 5978.0 nM 5.22