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Assay Detail

CHEMBL5577176

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BRD4 BD1 (unknown origin)
18
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a potent orally available pyrazolopyridone derivative as a novel selective bromodomain and extra-terminal domain (BET)-first bromodomain (BD1) inhibitor.
Hagihara S, Ishizawa K, Soga K, Honjo T, Takai S, Kawano Y, Kikuchi M, Nishidate A, Matsumoto F, Murase M, Hashimoto N, Sasaki C, Miyaguchi I, Okada O, Akashi T, Nakayama S, Ogasawara Y, Endo J.
Bioorg Med Chem Lett (2024) 109 : 129849 -129849
PubMed 38876177 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.08 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5584084 3 8.30
CHEMBL5590440 1 7.70
CHEMBL5591882 2 7.68
CHEMBL5594175 2 7.58
CHEMBL5584185 1 7.31
CHEMBL1957266 1 7.07
CHEMBL5593182 1 7.01
CHEMBL5590067 1 6.92
CHEMBL5589915 1 6.85
CHEMBL5591546 1 6.82
CHEMBL5590578 1 6.75
CHEMBL5590838 1 6.66
CHEMBL5591177 1 6.62
CHEMBL5590811 1 5.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5584084 IC50 = 5.0 nM 8.30
CHEMBL5590440 IC50 = 20.0 nM 7.70
CHEMBL5584084 IC50 = 21.0 nM 7.68
CHEMBL5591882 IC50 = 21.0 nM 7.68
CHEMBL5594175 IC50 = 26.0 nM 7.58
CHEMBL5584185 IC50 = 49.0 nM 7.31
CHEMBL1957266 IC50 = 85.0 nM 7.07
CHEMBL5593182 IC50 = 98.0 nM 7.01
CHEMBL5591882 IC50 = 98.0 nM 7.01
CHEMBL5590067 IC50 = 120.0 nM 6.92
CHEMBL5594175 IC50 = 130.0 nM 6.89
CHEMBL5589915 IC50 = 140.0 nM 6.85
CHEMBL5591546 IC50 = 150.0 nM 6.82
CHEMBL5584084 IC50 = 180.0 nM 6.75
CHEMBL5590578 IC50 = 180.0 nM 6.75
CHEMBL5590838 IC50 = 220.0 nM 6.66
CHEMBL5591177 IC50 = 240.0 nM 6.62
CHEMBL5590811 IC50 = 1600.0 nM 5.80