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Assay Detail

CHEMBL5577226

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 6His-tagged human UCHL1 using Ub-Rh110Gly as fluorogenic substrate preincubated for 1 hr followed by substrate addition by plate reader analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (CHEMBL6159)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design and Synthesis of Covalent Inhibitors for Deubiquitinase and Acetyltransferase ChlaDUB1 of <i>Chlamydia trachomatis</i>.
Zimmermann T, Feng J, de Campos LJ, Knight LA, Schlötzer J, Ramirez YA, Schwickert K, Zehe M, Adler TB, Schirmeister T, Kisker C, Sotriffer C, Conda-Sheridan M, Decker M.
J Med Chem (2024) 67 : 10710 -10742
PubMed 38897928 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.34 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5589657 1 7.30
CHEMBL5589694 1 5.38
CHEMBL5590899 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5589657 IC50 = 50.0 nM 7.30
CHEMBL5589694 IC50 = 4200.0 nM 5.38
CHEMBL5590899 IC50 < 1000.0 nM -