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Assay Detail

CHEMBL5578005

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PRMT5 in human HCT-116 cells with MTAP KO assessed as decrease in SMDA level incubated for 48 hrs by immunofluorescence analysis
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HCT-116
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein arginine N-methyltransferase 5 (CHEMBL1795116)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and <i>In Vivo</i> Efficacy of AZ-PRMT5i-1, a Novel PRMT5 Inhibitor with High MTA Cooperativity.
Smith JM, Barlaam B, Beattie D, Bradshaw L, Chan HM, Chiarparin E, Collingwood O, Cooke SL, Cronin A, Cumming I, Dean E, Debreczeni JÉ, Del Barco Barrantes I, Diene C, Gianni D, Guerot C, Guo X, Guven S, Hayhow TG, Hong T, Kemmitt PD, Lamont GM, Lamont S, Lynch JT, McWilliams L, Moore S, Raubo P, Robb GR, Robinson J, Scott JS, Srinivasan B, Steward O, Stubbs CJ, Syson K, Tan L, Turner O, Underwood E, Urosevic J, Vazquez-Chantada M, Whittaker AL, Wilson DM, Winter-Holt JJ.
J Med Chem (2024) 67 : 13604 -13638
PubMed 39080842 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 6.65 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5589904 1 8.40
CHEMBL5593070 1 8.40
CHEMBL5594655 1 8.30
CHEMBL5594567 1 8.22
CHEMBL5583712 1 7.22
CHEMBL5592096 1 7.18
CHEMBL5591445 1 7.12
CHEMBL5590720 1 6.85
CHEMBL5593804 1 6.82
CHEMBL5594801 1 6.62
CHEMBL5590589 1 6.52
CHEMBL5583660 1 6.42
CHEMBL5584160 1 6.42
CHEMBL5590816 1 6.24
CHEMBL5594198 1 6.18
CHEMBL5593457 1 6.17
CHEMBL5591144 1 5.89
CHEMBL5593058 1 5.32
CHEMBL5591780 1 5.19
CHEMBL5593194 1 5.11
CHEMBL5593534 1 5.10
CHEMBL5589874 1 -
CHEMBL5594285 1 -
CHEMBL5590674 1 -
CHEMBL5590521 1 -
CHEMBL5592639 1 -
CHEMBL5584072 1 -
CHEMBL5593993 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5589904 IC50 = 4.0 nM 8.40
CHEMBL5593070 IC50 = 4.0 nM 8.40
CHEMBL5594655 IC50 = 5.0 nM 8.30
CHEMBL5594567 IC50 = 6.0 nM 8.22
CHEMBL5583712 IC50 = 60.0 nM 7.22
CHEMBL5592096 IC50 = 66.0 nM 7.18
CHEMBL5591445 IC50 = 76.0 nM 7.12
CHEMBL5590720 IC50 = 140.0 nM 6.85
CHEMBL5593804 IC50 = 150.0 nM 6.82
CHEMBL5594801 IC50 = 240.0 nM 6.62
CHEMBL5590589 IC50 = 300.0 nM 6.52
CHEMBL5583660 IC50 = 380.0 nM 6.42
CHEMBL5584160 IC50 = 380.0 nM 6.42
CHEMBL5590816 IC50 = 580.0 nM 6.24
CHEMBL5594198 IC50 = 660.0 nM 6.18
CHEMBL5593457 IC50 = 680.0 nM 6.17
CHEMBL5591144 IC50 = 1300.0 nM 5.89
CHEMBL5593058 IC50 = 4800.0 nM 5.32
CHEMBL5591780 IC50 = 6400.0 nM 5.19
CHEMBL5593194 IC50 = 7800.0 nM 5.11
CHEMBL5593534 IC50 = 7900.0 nM 5.10
CHEMBL5589874 IC50 > 30000.0 nM -
CHEMBL5594285 IC50 > 30000.0 nM -
CHEMBL5590674 IC50 > 10000.0 nM -
CHEMBL5590521 IC50 > 30000.0 nM -
CHEMBL5592639 IC50 > 30000.0 nM -
CHEMBL5584072 IC50 > 30000.0 nM -
CHEMBL5593993 IC50 > 30000.0 nM -