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Assay Detail

CHEMBL5579430

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of STT3A/STT3B mediated N-linked glycosylation in STT3A knockout human H1-HeLa cells stably transduced with Halo3N-ER sequence by measuring effective concentration incubated for 24 hrs by Western blotting analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type H1-HeLa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of Potent STT3A/B Inhibitors and Assessment of Their Multipathogen Antiviral Potential and Safety.
Pero JE, Mueller EA, Adams AM, Adolph RS, Bagchi P, Balce D, Bantscheff M, Barauskas O, Bartha I, Bohan D, Cai H, Carabajal E, Cassidy J, Cato M, Chaudhary KW, Chen D, Chen YP, Colas C, Darwech I, Eberl HC, Fernandez B, Gordon E, Grosse J, Hansen J, Hetzler B, Hwang S, Jeyasingh S, Kowalski B, Lehmann S, Lo G, McAllaster M, McHugh C, Momont C, Newby Z, Nigro M, Oladunni F, Pannirselvam M, Park A, Pearson N, Peat AJ, Plastridge B, Ranjan R, Safabakhsh P, Shapiro ND, Soriaga L, Stokes N, Sweeney D, Talecki L, Telenti A, Terrell A, Tse W, Wang L, Wang S, Wedel L, Werner T, Dalmas Wilk D, Yim S, Zhou J.
J Med Chem (2024) 67 : 14586 -14608
PubMed 39136957 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 7.41 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5584184 1 7.55
CHEMBL5583722 1 7.47
CHEMBL5591147 1 7.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5584184 EC50 = 28.0 nM 7.55
CHEMBL5583722 EC50 = 34.0 nM 7.47
CHEMBL5591147 EC50 = 63.0 nM 7.20