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Assay Detail

CHEMBL5582966

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human G4-quadruplex Pu27 DNA 5'- TGGGGAGGGTGGGGAGGGTGGGGAAGG-3' assessed as change in fluorescence intensity at 1 uM incubated for 0.5 hrs in presence of different concentrations of DNA by fluorescence spectra analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Target

quadruplex DNA (CHEMBL3832742)
Type NUCLEIC-ACID
Organism Homo sapiens

Publication

A Comparative Study on the DNA Interactions and Biological Activities of Benzophenanthridine and Protoberberine Alkaloids.
Zu S, Long Z, Zhang X, Sheng J, Xu Y, Sun H, Liu X, Shangguan D.
J Nat Prod (2024) 87 : 2170 -2179
PubMed 39213483 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 6 5.64 7.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5594821 1 7.40
CHEMBL362071 COPTISINE 1 5.66
CHEMBL13045 CHELERYTHRINE 1 5.28
CHEMBL295124 BERBERINE 4.0 1 5.27
CHEMBL417799 SANGUINARIUM 2.0 1 5.14
CHEMBL5593587 1 5.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5594821 Kd = 40.0 nM 7.40
CHEMBL362071 COPTISINE Kd = 2170.0 nM 5.66
CHEMBL13045 CHELERYTHRINE Kd = 5290.0 nM 5.28
CHEMBL295124 BERBERINE Kd = 5400.0 nM 5.27
CHEMBL417799 SANGUINARIUM Kd = 7190.0 nM 5.14
CHEMBL5593587 Kd = 8040.0 nM 5.09