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Assay Detail

CHEMBL5583007

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of ThT from human G4-quadruplex Bcl2 DNA 5'- CGGGCGCGGGAGGAAGGGGGCGGGAGC-3' incubated for 0.5 hrs by SpectraMax M2 microplate reader analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Target

quadruplex DNA (CHEMBL3832742)
Type NUCLEIC-ACID
Organism Homo sapiens

Publication

A Comparative Study on the DNA Interactions and Biological Activities of Benzophenanthridine and Protoberberine Alkaloids.
Zu S, Long Z, Zhang X, Sheng J, Xu Y, Sun H, Liu X, Shangguan D.
J Nat Prod (2024) 87 : 2170 -2179
PubMed 39213483 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 6 6.11 6.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL362071 COPTISINE 1 6.46
CHEMBL417799 SANGUINARIUM 2.0 1 6.36
CHEMBL5593587 1 6.18
CHEMBL13045 CHELERYTHRINE 1 6.07
CHEMBL176008 NITIDINE 1 5.82
CHEMBL295124 BERBERINE 4.0 1 5.74

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL362071 COPTISINE Kd = 350.0 nM 6.46
CHEMBL417799 SANGUINARIUM Kd = 440.0 nM 6.36
CHEMBL5593587 Kd = 660.0 nM 6.18
CHEMBL13045 CHELERYTHRINE Kd = 860.0 nM 6.07
CHEMBL176008 NITIDINE Kd = 1520.0 nM 5.82
CHEMBL295124 BERBERINE Kd = 1840.0 nM 5.74