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Assay Detail

CHEMBL5585102

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ALKBH5 (unknown origin) expressed in Escherichia coli BL21 (DE3) using 5'-ATTGTCA(m6A)CAGCAGA-FAM-3 as substrate pre-incubated for 30 mins followed by substrate addition and measured for 1 hrs by FP based analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RNA demethylase ALKBH5 (CHEMBL3621037)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small molecules that regulate the N<sup>6</sup>-methyladenosine RNA modification as potential anti-cancer agents.
Harrahill NJ, Hadden MK.
Eur J Med Chem (2024) 274 : 116526 -116526
PubMed 38805939 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 5.05 5.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL418068 RHEIN 1 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL418068 RHEIN IC50 = 9000.0 nM 5.05