Assay Detail
Binding
CHEMBL5585111
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of FTO (unknown origin) expressed in Escherichia coli BL21(DE3) using 5'-TAGACATTGCCATTCTCGATAGG(m6A)-TCCGGTCAAACCTAGACGAATTCCA-3' as substrate by FP assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Alpha-ketoglutarate-dependent dioxygenase FTO (CHEMBL2331065) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Small molecules that regulate the N<sup>6</sup>-methyladenosine RNA modification as potential anti-cancer agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 5.29 | 5.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL41286 | DIACEREIN | 3.0 | 1 | 5.82 |
| CHEMBL5281651 | — | — | 1 | 5.82 |
| CHEMBL426560 | — | — | 1 | 5.55 |
| CHEMBL1230640 | — | — | 1 | 5.48 |
| CHEMBL953 | ENTACAPONE | 4.0 | 1 | 5.46 |
| CHEMBL3989553 | NAFAMOSTAT MESYLATE | 3.0 | 1 | 4.86 |
| CHEMBL414883 | RADICICOL | — | 1 | 4.80 |
| CHEMBL235934 | CLAUSINE E | — | 1 | 4.55 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL41286 | DIACEREIN | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL5281651 | — | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL426560 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL1230640 | — | IC50 | = | 3300.0 | nM | 5.48 |
| CHEMBL953 | ENTACAPONE | IC50 | = | 3500.0 | nM | 5.46 |
| CHEMBL3989553 | NAFAMOSTAT MESYLATE | IC50 | = | 13800.0 | nM | 4.86 |
| CHEMBL414883 | RADICICOL | IC50 | = | 16000.0 | nM | 4.80 |
| CHEMBL235934 | CLAUSINE E | IC50 | = | 28000.0 | nM | 4.55 |