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Assay Detail

CHEMBL5585900

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PD-1/PD-L1 interaction in human Jurkat T cells co-cultured with CHO/TCRAct/PDL1 cells assessed as restoration of T cell activation measured for 6 hrs by luminescence based immune checkpoint blockade assay
5
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

N-methylmorpholine incorporation into the structure of biphenyl leads to the bioactive inhibitor of PD-1/PD-L1 interaction.
Zaber J, Skalniak L, Gudz GP, Hec-Gałązka A, Zarnik M, Tyrcha U, Stec M, Siedlar M, Holak TA, Sitar T, Muszak D.
Bioorg Med Chem Lett (2024) 110 : 129882 -129882
PubMed 38996937 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 5.85 7.77
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5398278 1 7.77
CHEMBL5078455 1 5.18
CHEMBL5597858 2 4.59
CHEMBL5597887 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5398278 EC50 = 17.0 nM 7.77
CHEMBL5078455 EC50 = 6600.0 nM 5.18
CHEMBL5597858 EC50 = 25800.0 nM 4.59
CHEMBL5597858 Inhibition - % -
CHEMBL5597887 Inhibition - % -