Assay Detail
Binding
CHEMBL5587984
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Displacement of dansylamide from human recombinant CA II expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by fluorescence microplate reader
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Investigating Active Site Binding of Ligands to High and Low Activity Carbonic Anhydrase Enzymes Using Native Mass Spectrometry.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 7 | 6.40 | 7.80 |
Compounds Tested
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL118 | CELECOXIB | Kd | = | 15.8 | nM | 7.80 |
| CHEMBL20 | ACETAZOLAMIDE | Kd | = | 20.0 | nM | 7.70 |
| CHEMBL19 | METHAZOLAMIDE | Kd | = | 54.8 | nM | 7.26 |
| CHEMBL406 | INDAPAMIDE | Kd | = | 1420.0 | nM | 5.85 |
| CHEMBL1054 | TRICHLORMETHIAZIDE | Kd | = | 2600.0 | nM | 5.58 |
| CHEMBL35 | FUROSEMIDE | Kd | = | 3140.0 | nM | 5.50 |
| CHEMBL435 | HYDROCHLOROTHIAZIDE | Kd | = | 7190.0 | nM | 5.14 |