Assay Detail
Functional
CHEMBL5604069
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Functional biochemical assay to identify treatments for viral infectious disease that targets Dengue virus type 2 NS2B-NS3 protease. Utilizing a direct enzyme activity measurement method, reading the fluorescence intensity is used to characterize the relative potency of inhibitors as IC50. Synthetic construct has been made based on Dengue virus type 2 (strain Thailand/16681/1984) by Centre for Medicines Discovery, University of Oxford, and the assay has been performed by Weizmann Institute of Science.The protocol is publicly available: dx.doi.org/10.17504/protocols.io.q26g7yebkgwz/v3
3030
Total Activities
2766
Compounds Tested
1
Activity Types
1
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | dengue virus type 2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
ASAP - DENV-2 NS2B-NS3 protease fluorescence dose response
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3030 | 4.70 | 6.63 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| Construct protein sequence | Construct protein sequence | - | — | SMADLELERAADVKWEDQAEISGSSPILSITISEDGSMSIKNEEEEQTLGGGGSGGGGAGVLWDVPSPPPMGKAELEDGAYRIKQKGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHKGKRIEPSWADVKKDLISYGGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTNAGTIGAVSLDFSPGTSGSPIIDKKGKVVGLYGNGVVTRSGAYVSAIAQTEKSIEDNPEIEDDIFRK |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3919119 | — | — | 24 | 6.63 |
| CHEMBL3740727 | — | — | 64 | 6.19 |
| CHEMBL5606223 | — | — | 2 | 5.75 |
| CHEMBL5604424 | — | — | 7 | 5.73 |
| CHEMBL5604726 | — | — | 2 | 5.70 |
| CHEMBL5598684 | — | — | 2 | 5.63 |
| CHEMBL5605372 | — | — | 2 | 5.61 |
| CHEMBL5604470 | — | — | 2 | 5.60 |
| CHEMBL5605466 | — | — | 1 | 5.58 |
| CHEMBL5606070 | — | — | 1 | 5.48 |
| CHEMBL5605103 | — | — | 2 | 5.47 |
| CHEMBL569561 | — | — | 1 | 5.46 |
| CHEMBL5605003 | — | — | 1 | 5.42 |
| CHEMBL5605782 | — | — | 1 | 5.40 |
| CHEMBL5599838 | — | — | 3 | 5.39 |
| CHEMBL5604914 | — | — | 2 | 5.37 |
| CHEMBL5605274 | — | — | 2 | 5.37 |
| CHEMBL5605982 | — | — | 2 | 5.37 |
| CHEMBL5605533 | — | — | 3 | 5.37 |
| CHEMBL5605664 | — | — | 2 | 5.36 |
| CHEMBL5604989 | — | — | 2 | 5.33 |
| CHEMBL5604216 | — | — | 2 | 5.32 |
| CHEMBL5605565 | — | — | 1 | 5.29 |
| CHEMBL5606145 | — | — | 1 | 5.29 |
| CHEMBL5605516 | — | — | 2 | 5.29 |
| CHEMBL5604531 | — | — | 2 | 5.28 |
| CHEMBL5604806 | — | — | 2 | 5.26 |
| CHEMBL5606200 | — | — | 2 | 5.25 |
| CHEMBL5605472 | — | — | 3 | 5.24 |
| CHEMBL5605298 | — | — | 3 | 5.24 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3919119 | — | IC50 | = | 236.0 | nM | 6.63 |
| CHEMBL3919119 | — | IC50 | = | 265.0 | nM | 6.58 |
| CHEMBL3919119 | — | IC50 | = | 270.0 | nM | 6.57 |
| CHEMBL3919119 | — | IC50 | = | 274.0 | nM | 6.56 |
| CHEMBL3919119 | — | IC50 | = | 276.0 | nM | 6.56 |
| CHEMBL3919119 | — | IC50 | = | 310.0 | nM | 6.51 |
| CHEMBL3919119 | — | IC50 | = | 397.0 | nM | 6.40 |
| CHEMBL3919119 | — | IC50 | = | 474.0 | nM | 6.32 |
| CHEMBL3919119 | — | IC50 | = | 507.0 | nM | 6.29 |
| CHEMBL3919119 | — | IC50 | = | 572.0 | nM | 6.24 |
| CHEMBL3919119 | — | IC50 | = | 583.0 | nM | 6.23 |
| CHEMBL3919119 | — | IC50 | = | 585.0 | nM | 6.23 |
| CHEMBL3740727 | — | IC50 | = | 645.0 | nM | 6.19 |
| CHEMBL3919119 | — | IC50 | = | 720.0 | nM | 6.14 |
| CHEMBL3919119 | — | IC50 | = | 732.0 | nM | 6.13 |
| CHEMBL3919119 | — | IC50 | = | 818.0 | nM | 6.09 |
| CHEMBL3740727 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL3740727 | — | IC50 | = | 924.0 | nM | 6.03 |
| CHEMBL3740727 | — | IC50 | = | 977.0 | nM | 6.01 |
| CHEMBL3740727 | — | IC50 | = | 1022.0 | nM | 5.99 |
| CHEMBL3740727 | — | IC50 | = | 1083.0 | nM | 5.96 |
| CHEMBL3740727 | — | IC50 | = | 1132.0 | nM | 5.95 |
| CHEMBL3740727 | — | IC50 | = | 1146.0 | nM | 5.94 |
| CHEMBL3740727 | — | IC50 | = | 1151.0 | nM | 5.94 |
| CHEMBL3740727 | — | IC50 | = | 1162.0 | nM | 5.93 |
| CHEMBL3740727 | — | IC50 | = | 1171.0 | nM | 5.93 |
| CHEMBL3740727 | — | IC50 | = | 1176.0 | nM | 5.93 |
| CHEMBL3740727 | — | IC50 | = | 1227.0 | nM | 5.91 |
| CHEMBL3740727 | — | IC50 | = | 1219.0 | nM | 5.91 |
| CHEMBL3740727 | — | IC50 | = | 1243.0 | nM | 5.91 |
| CHEMBL3740727 | — | IC50 | = | 1240.0 | nM | 5.91 |
| CHEMBL3740727 | — | IC50 | = | 1220.0 | nM | 5.91 |
| CHEMBL3740727 | — | IC50 | = | 1219.0 | nM | 5.91 |
| CHEMBL3740727 | — | IC50 | = | 1250.0 | nM | 5.90 |
| CHEMBL3740727 | — | IC50 | = | 1264.0 | nM | 5.90 |
| CHEMBL3740727 | — | IC50 | = | 1254.0 | nM | 5.90 |
| CHEMBL3740727 | — | IC50 | = | 1270.0 | nM | 5.90 |
| CHEMBL3740727 | — | IC50 | = | 1269.0 | nM | 5.90 |
| CHEMBL3740727 | — | IC50 | = | 1266.0 | nM | 5.90 |
| CHEMBL3740727 | — | IC50 | = | 1249.0 | nM | 5.90 |
| CHEMBL3740727 | — | IC50 | = | 1301.0 | nM | 5.89 |
| CHEMBL3740727 | — | IC50 | = | 1306.0 | nM | 5.88 |
| CHEMBL3740727 | — | IC50 | = | 1315.0 | nM | 5.88 |
| CHEMBL3740727 | — | IC50 | = | 1334.0 | nM | 5.88 |
| CHEMBL3740727 | — | IC50 | = | 1365.0 | nM | 5.87 |
| CHEMBL3740727 | — | IC50 | = | 1342.0 | nM | 5.87 |
| CHEMBL3740727 | — | IC50 | = | 1336.0 | nM | 5.87 |
| CHEMBL3740727 | — | IC50 | = | 1376.0 | nM | 5.86 |
| CHEMBL3740727 | — | IC50 | = | 1381.0 | nM | 5.86 |
| CHEMBL3740727 | — | IC50 | = | 1437.0 | nM | 5.84 |