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Assay Detail

CHEMBL5609910

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant HDAC3 using Boc-Lys (acetyl)-AMC as flurogenic substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by fluorescence assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 3 (CHEMBL1829)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological activity evaluation of novel HDAC3 selective inhibitors for combination with Venetoclax against acute myeloid leukemia.
Liu E, Chen Y, Qin M, Yue K, Sun S, Jiang Y, Li X.
Eur J Med Chem (2024) 276 : 116663 -116663
PubMed 39047608 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.69 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4597954 1 9.52
CHEMBL5613686 1 9.30
CHEMBL5613071 1 8.62
CHEMBL5613673 1 8.62
CHEMBL5614136 1 8.51
CHEMBL5614363 1 8.38
CHEMBL5612659 1 8.34
CHEMBL5613525 1 8.05
CHEMBL5612950 1 7.95
CHEMBL5613340 1 7.69
CHEMBL5611904 1 7.49
CHEMBL5612403 1 7.40
CHEMBL3621988 TUCIDINOSTAT 3.0 1 7.20
CHEMBL5613070 1 7.09
CHEMBL5612071 1 6.97
CHEMBL5612582 1 6.91
CHEMBL5613949 1 6.87
CHEMBL5613741 1 6.77
CHEMBL5612263 1 6.76
CHEMBL5613057 1 6.57
CHEMBL5612404 1 6.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4597954 IC50 = 0.3 nM 9.52
CHEMBL5613686 IC50 = 0.5 nM 9.30
CHEMBL5613071 IC50 = 2.4 nM 8.62
CHEMBL5613673 IC50 = 2.4 nM 8.62
CHEMBL5614136 IC50 = 3.1 nM 8.51
CHEMBL5614363 IC50 = 4.2 nM 8.38
CHEMBL5612659 IC50 = 4.6 nM 8.34
CHEMBL5613525 IC50 = 9.0 nM 8.05
CHEMBL5612950 IC50 = 11.3 nM 7.95
CHEMBL5613340 IC50 = 20.4 nM 7.69
CHEMBL5611904 IC50 = 32.3 nM 7.49
CHEMBL5612403 IC50 = 39.5 nM 7.40
CHEMBL3621988 TUCIDINOSTAT IC50 = 63.3 nM 7.20
CHEMBL5613070 IC50 = 80.4 nM 7.09
CHEMBL5612071 IC50 = 106.8 nM 6.97
CHEMBL5612582 IC50 = 124.3 nM 6.91
CHEMBL5613949 IC50 = 135.6 nM 6.87
CHEMBL5613741 IC50 = 169.6 nM 6.77
CHEMBL5612263 IC50 = 171.6 nM 6.76
CHEMBL5613057 IC50 = 268.2 nM 6.57
CHEMBL5612404 IC50 = 362.5 nM 6.44