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Assay Detail

CHEMBL5622977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of alpha-amylase (unknown origin) using starch as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by absorbance based assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Alpha amylase (CHEMBL6066863)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

SAR and lead optimization of (Z)-5-(4-hydroxy-3-methoxybenzylidene)-3-(2-morpholinoacetyl)thiazolidine-2,4-dione as a potential multi-target antidiabetic agent.
Shah M, Jan MS, Sadiq A, Khan S, Rashid U.
Eur J Med Chem (2023) 258 : 115591 -115591
PubMed 37393789 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 5.40 6.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5624535 1 6.72
CHEMBL5624476 1 6.70
CHEMBL5630676 1 6.62
CHEMBL5624847 1 6.61
CHEMBL5631075 1 6.54
CHEMBL5630566 1 6.50
CHEMBL5624515 1 6.43
CHEMBL5631021 1 6.40
CHEMBL5630180 1 6.05
CHEMBL5624539 1 6.02
CHEMBL404271 ACARBOSE 4.0 1 4.99
CHEMBL5624654 1 4.74
CHEMBL5624938 1 4.70
CHEMBL5630880 1 4.55
CHEMBL5630847 1 4.53
CHEMBL5629890 1 4.45
CHEMBL5630679 1 4.29
CHEMBL5630282 1 4.25
CHEMBL5630820 1 4.24
CHEMBL5630400 1 4.05
CHEMBL5624477 1 4.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5624535 IC50 = 190.0 nM 6.72
CHEMBL5624476 IC50 = 201.0 nM 6.70
CHEMBL5630676 IC50 = 241.0 nM 6.62
CHEMBL5624847 IC50 = 243.0 nM 6.61
CHEMBL5631075 IC50 = 287.0 nM 6.54
CHEMBL5630566 IC50 = 319.0 nM 6.50
CHEMBL5624515 IC50 = 367.0 nM 6.43
CHEMBL5631021 IC50 = 401.0 nM 6.40
CHEMBL5630180 IC50 = 890.0 nM 6.05
CHEMBL5624539 IC50 = 957.0 nM 6.02
CHEMBL404271 ACARBOSE IC50 = 10320.0 nM 4.99
CHEMBL5624654 IC50 = 18410.0 nM 4.74
CHEMBL5624938 IC50 = 20140.0 nM 4.70
CHEMBL5630880 IC50 = 28000.0 nM 4.55
CHEMBL5630847 IC50 = 29440.0 nM 4.53
CHEMBL5629890 IC50 = 35170.0 nM 4.45
CHEMBL5630679 IC50 = 51250.0 nM 4.29
CHEMBL5630282 IC50 = 55670.0 nM 4.25
CHEMBL5630820 IC50 = 58170.0 nM 4.24
CHEMBL5630400 IC50 = 89710.0 nM 4.05
CHEMBL5624477 IC50 = 94080.0 nM 4.03