Assay Detail
Binding
CHEMBL5627085
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of SARS-CoV-2 Main protease expressed in Escherichia coli BL21 (DE3) using MCA-AVLQSGFR-Lys (DNP)-Lys-NH2 as substrate preincubated for 10 mins followed by substrate addition and measured for 10 mins by FRET assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1ab (CHEMBL4523582) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
Publication
Discovery and structure-activity relationship studies of novel α-ketoamide derivatives targeting the SARS-CoV-2 main protease.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 7.80 | 7.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5195325 | — | — | 1 | 7.82 |
| CHEMBL5270743 | — | — | 1 | 7.77 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5195325 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL5270743 | — | IC50 | = | 17.0 | nM | 7.77 |