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Assay Detail

CHEMBL5628447

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Binding
Inhibition of N-terminal polyHis-tagged human RIPK3 (1 to 307 residues) expressed in Sf9 cells using MBP as substrate preincubated for 15 mins followed by ATP addition measured after 90 mins by ADP-Glo assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Exploring positions 6 and 7 of a quinazoline-based scaffold leads to changes in selectivity and potency towards RIPK2/3 kinases.
Misehe M, Matoušová M, Dvořáková A, Hercík K, Škach K, Chalupská D, Dejmek M, Šála M, Hájek M, Boura E, Mertlíková-Kaiserová H, Nencka R.
Eur J Med Chem (2023) 260 : 115717 -115717
PubMed 37598483 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.14 7.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4779280 1 7.11
CHEMBL5631858 1 6.93
CHEMBL5632745 1 6.91
CHEMBL5632238 1 6.85
CHEMBL5632098 1 6.42
CHEMBL5633099 1 6.41
CHEMBL5632272 1 6.40
CHEMBL5633841 1 6.33
CHEMBL5631246 1 6.26
CHEMBL5632081 1 5.82
CHEMBL5633859 1 5.80
CHEMBL5632322 1 5.72
CHEMBL5633433 1 5.68
CHEMBL5631295 1 5.58
CHEMBL5632922 1 5.54
CHEMBL5633010 1 5.44
CHEMBL5632677 1 5.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4779280 IC50 = 77.0 nM 7.11
CHEMBL5631858 IC50 = 117.0 nM 6.93
CHEMBL5632745 IC50 = 122.0 nM 6.91
CHEMBL5632238 IC50 = 141.0 nM 6.85
CHEMBL5632098 IC50 = 382.0 nM 6.42
CHEMBL5633099 IC50 = 388.0 nM 6.41
CHEMBL5632272 IC50 = 398.0 nM 6.40
CHEMBL5633841 IC50 = 464.0 nM 6.33
CHEMBL5631246 IC50 = 551.0 nM 6.26
CHEMBL5632081 IC50 = 1500.0 nM 5.82
CHEMBL5633859 IC50 = 1600.0 nM 5.80
CHEMBL5632322 IC50 = 1900.0 nM 5.72
CHEMBL5633433 IC50 = 2100.0 nM 5.68
CHEMBL5631295 IC50 = 2600.0 nM 5.58
CHEMBL5632922 IC50 = 2900.0 nM 5.54
CHEMBL5633010 IC50 = 3600.0 nM 5.44
CHEMBL5632677 IC50 = 6000.0 nM 5.22