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Assay Detail

CHEMBL5628569

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human LPA1 receptor stably expressed in rat RH7777 cells assessed as reduction in LPA-induced intracellular Ca2+ concentration preincubated for 30 mins followed by LPA addition measured by Fluo-4 probe based fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type RH7777
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysophosphatidic acid receptor 1 (CHEMBL3819)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Lead generation from N-[benzyl(4-phenylbutyl)carbamoyl]amino acid as a novel LPA<sub>1</sub> antagonist for the treatment of systemic sclerosis.
Kobayashi Y, Uneuchi F, Naruse T, Matsuda D, Okumura-Kitajima L, Kajiyama H, Wada R, Yonemoto Y, Nakano K, Toki H, Kamigaso S, Yamagishi J, Tokura S, Kakinuma H, Kuroda S.
Eur J Med Chem (2023) 260 : 115749 -115749
PubMed 37639822 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.13 8.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5633614 1 8.80
CHEMBL5632303 1 7.52
CHEMBL5632315 1 7.29
CHEMBL5632273 1 6.96
CHEMBL5633770 1 6.33
CHEMBL5633672 1 6.28
CHEMBL5631989 1 6.23
CHEMBL5633208 1 5.75
CHEMBL5633075 1 5.67
CHEMBL5632224 1 5.67
CHEMBL5631605 1 5.56
CHEMBL5634050 1 5.56
CHEMBL5633162 1 5.46
CHEMBL5633792 1 5.44
CHEMBL5631516 1 5.31
CHEMBL5633356 1 5.30
CHEMBL5632524 1 5.06
CHEMBL5633769 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5633614 IC50 = 1.6 nM 8.80
CHEMBL5632303 IC50 = 30.1 nM 7.52
CHEMBL5632315 IC50 = 51.4 nM 7.29
CHEMBL5632273 IC50 = 110.0 nM 6.96
CHEMBL5633770 IC50 = 470.0 nM 6.33
CHEMBL5633672 IC50 = 521.0 nM 6.28
CHEMBL5631989 IC50 = 586.0 nM 6.23
CHEMBL5633208 IC50 = 1790.0 nM 5.75
CHEMBL5633075 IC50 = 2140.0 nM 5.67
CHEMBL5632224 IC50 = 2150.0 nM 5.67
CHEMBL5631605 IC50 = 2750.0 nM 5.56
CHEMBL5634050 IC50 = 2770.0 nM 5.56
CHEMBL5633162 IC50 = 3480.0 nM 5.46
CHEMBL5633792 IC50 = 3660.0 nM 5.44
CHEMBL5631516 IC50 = 4890.0 nM 5.31
CHEMBL5633356 IC50 = 5040.0 nM 5.30
CHEMBL5632524 IC50 = 8770.0 nM 5.06
CHEMBL5633769 IC50 > 10000.0 nM -