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Assay Detail

CHEMBL5649629

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of kinase tracer 236 from GST-tagged Axl (unknown origin) assessed as inhibition constant incubated for 1 hr by HTRF based TR-FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
4
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase receptor UFO (CHEMBL4895)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.
Paolino M, Choidas A, Wallner S, Pranjic B, Uribesalgo I, Loeser S, Jamieson AM, Langdon WY, Ikeda F, Fededa JP, Cronin SJ, Nitsch R, Schultz-Fademrecht C, Eickhoff J, Menninger S, Unger A, Torka R, Gruber T, Hinterleitner R, Baier G, Wolf D, Ullrich A, Klebl BM, Penninger JM.
Nature (2014) 507 : 508 -512
PubMed 24553136 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.54 7.54

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_ID EFO_ID - EFO:0003869
EFO_TERM EFO_TERM - cancer
EFO_TERM EFO_TERM - breast neoplasm

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3808844 1 7.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3808844 IC50 = 29.0 nM 7.54