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Assay Detail

CHEMBL5654256

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat FAAH assessed as kinact/Ki ratio incubated for 15 min in presence of glutamate dehydrogenase by 384-well format microplate reader assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL3229)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Mechanistic and pharmacological characterization of PF-04457845: a highly potent and selective fatty acid amide hydrolase inhibitor that reduces inflammatory and noninflammatory pain.
Ahn K, Smith SE, Liimatta MB, Beidler D, Sadagopan N, Dudley DT, Young T, Wren P, Zhang Y, Swaney S, Van Becelaere K, Blankman JL, Nomura DK, Bhattachar SN, Stiff C, Nomanbhoy TK, Weerapana E, Johnson DS, Cravatt BF.
J Pharmacol Exp Ther (2011) 338 : 114 -124
PubMed 21505060 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.57 7.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1651534 REDAFAMDASTAT 2.0 1 7.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1651534 REDAFAMDASTAT IC50 = 26.7 nM 7.57