Assay Detail
Binding
CHEMBL5659524
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of PRMT5 in human ZR-75-1 cells assessed as decrease in SDMA level incubated for 3 days
1
Total Activities
1
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | ZR-75-1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Protein arginine N-methyltransferase 5 (CHEMBL1795116) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Activation of the p53-MDM4 regulatory axis defines the anti-tumour response to PRMT5 inhibition through its role in regulating cellular splicing.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 8.70 | 8.70 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_TERM | EFO_TERM | - | — | cancer |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4466233 | PEMRAMETOSTAT | 2.0 | 1 | 8.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4466233 | PEMRAMETOSTAT | EC50 | = | 2.0 | nM | 8.70 |