Assay Detail
Binding
CHEMBL5675948
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Inhibition of SMYD2 (unknown origin) using N-terminal GST-tagged MAP3K2 and 3H-SAM as substrate for 2 hrs by TopCount NXT plate reader
4
Total Activities
4
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Small molecule inhibitors and CRISPR/Cas9 mutagenesis demonstrate that SMYD2 and SMYD3 activity are dispensable for autonomous cancer cell proliferation.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.82 | 8.41 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_TERM | EFO_TERM | - | — | cancer |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5187983 | — | — | 1 | 8.41 |
| CHEMBL5197760 | — | — | 1 | 7.80 |
| CHEMBL3414623 | — | — | 1 | 7.77 |
| CHEMBL2169920 | — | — | 1 | 7.29 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5187983 | — | IC50 | = | 3.9 | nM | 8.41 |
| CHEMBL5197760 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL3414623 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL2169920 | — | IC50 | = | 51.0 | nM | 7.29 |