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Assay Detail

CHEMBL5681162

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against mouse BaF3 cells harbouring BCR-ABL1 Q252H mutant assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type BaF3
Confidence 1 — Organism
Curated By Autocuration

Target

BaF3 (CHEMBL614524)
Type CELL-LINE
Organism Mus musculus

Publication

Conformational control inhibition of the BCR-ABL1 tyrosine kinase, including the gatekeeper T315I mutant, by the switch-control inhibitor DCC-2036.
Chan WW, Wise SC, Kaufman MD, Ahn YM, Ensinger CL, Haack T, Hood MM, Jones J, Lord JW, Lu WP, Miller D, Patt WC, Smith BD, Petillo PA, Rutkoski TJ, Telikepalli H, Vogeti L, Yao T, Chun L, Clark R, Evangelista P, Gavrilescu LC, Lazarides K, Zaleskas VM, Stewart LJ, Van Etten RA, Flynn DL.
Cancer Cell (2011) 19 : 556 -568
PubMed 21481795 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.70 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1421 DASATINIB ANHYDROUS 4.0 1 9.52
CHEMBL255863 NILOTINIB 4.0 1 8.29
CHEMBL1738757 REBASTINIB 2.0 1 7.62
CHEMBL572878 TOZASERTIB 2.0 1 6.89
CHEMBL941 IMATINIB 4.0 1 6.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1421 DASATINIB ANHYDROUS IC50 = 0.3 nM 9.52
CHEMBL255863 NILOTINIB IC50 = 5.1 nM 8.29
CHEMBL1738757 REBASTINIB IC50 = 24.0 nM 7.62
CHEMBL572878 TOZASERTIB IC50 = 130.0 nM 6.89
CHEMBL941 IMATINIB IC50 = 700.0 nM 6.16