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Assay Detail

CHEMBL5683281

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGF-stimulated ERK5 autophosphorylation in human HeLa cells preincubated with compound for 1 hrs followed by EGF stimulation for 17 mins by SDS-PAGE analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 7 (CHEMBL5332)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural determinants for ERK5 (MAPK7) and leucine rich repeat kinase 2 activities of benzo[e]pyrimido-[5,4-b]diazepine-6(11H)-ones.
Deng X, Elkins JM, Zhang J, Yang Q, Erazo T, Gomez N, Choi HG, Wang J, Dzamko N, Lee JD, Sim T, Kim N, Alessi DR, Lizcano JM, Knapp S, Gray NS.
Eur J Med Chem (2013) 70 : 758 -767
PubMed 24239623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 22 6.34 7.10

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_TERM EFO_TERM - cancer

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5687018 1 7.10
CHEMBL2381340 1 7.05
CHEMBL1673043 1 6.80
CHEMBL1673039 1 6.72
CHEMBL1673053 1 6.70
CHEMBL1673051 1 6.70
CHEMBL1673052 1 6.64
CHEMBL1673040 1 6.62
CHEMBL5686992 1 6.62
CHEMBL1673046 1 6.62
CHEMBL1673041 1 6.58
CHEMBL2381339 1 6.58
CHEMBL1673047 1 6.51
CHEMBL1673048 1 6.50
CHEMBL1673057 1 5.91
CHEMBL5686989 1 5.88
CHEMBL5687020 1 5.51
CHEMBL5687005 1 5.39
CHEMBL5687010 1 5.33
CHEMBL1673056 1 4.94
CHEMBL4632948 1 -
CHEMBL1672987 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5687018 EC50 = 80.0 nM 7.10
CHEMBL2381340 EC50 = 90.0 nM 7.05
CHEMBL1673043 EC50 = 160.0 nM 6.80
CHEMBL1673039 EC50 = 190.0 nM 6.72
CHEMBL1673053 EC50 = 200.0 nM 6.70
CHEMBL1673051 EC50 = 200.0 nM 6.70
CHEMBL1673052 EC50 = 230.0 nM 6.64
CHEMBL1673040 EC50 = 240.0 nM 6.62
CHEMBL5686992 EC50 = 240.0 nM 6.62
CHEMBL1673046 EC50 = 240.0 nM 6.62
CHEMBL1673041 EC50 = 260.0 nM 6.58
CHEMBL2381339 EC50 = 260.0 nM 6.58
CHEMBL1673047 EC50 = 310.0 nM 6.51
CHEMBL1673048 EC50 = 320.0 nM 6.50
CHEMBL1673057 EC50 = 1240.0 nM 5.91
CHEMBL5686989 EC50 = 1320.0 nM 5.88
CHEMBL5687020 EC50 = 3100.0 nM 5.51
CHEMBL5687005 EC50 = 4110.0 nM 5.39
CHEMBL5687010 EC50 = 4680.0 nM 5.33
CHEMBL1673056 EC50 = 11380.0 nM 4.94
CHEMBL4632948 EC50 > 500.0 nM -
CHEMBL1672987 EC50 > 1000.0 nM -