Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL5683282

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal 6His-tagged human ERK5 expressed in baculovirus infected Sf21 cells co-expressing HA-tagged human MEK5-DD using ARKKRRHPSGPPTA peptide as substrate incubated for 20 mins in presence of [gamma-32P]-ATP by radiochemical assay
22
Total Activities
22
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 7 (CHEMBL5332)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural determinants for ERK5 (MAPK7) and leucine rich repeat kinase 2 activities of benzo[e]pyrimido-[5,4-b]diazepine-6(11H)-ones.
Deng X, Elkins JM, Zhang J, Yang Q, Erazo T, Gomez N, Choi HG, Wang J, Dzamko N, Lee JD, Sim T, Kim N, Alessi DR, Lizcano JM, Knapp S, Gray NS.
Eur J Med Chem (2013) 70 : 758 -767
PubMed 24239623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.43 7.18

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_TERM EFO_TERM - cancer

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1673051 1 7.18
CHEMBL5687018 1 7.09
CHEMBL1673039 1 7.06
CHEMBL1673052 1 7.01
CHEMBL1673043 1 6.94
CHEMBL5686992 1 6.85
CHEMBL1673053 1 6.84
CHEMBL2381340 1 6.79
CHEMBL1673047 1 6.77
CHEMBL2381339 1 6.70
CHEMBL1673041 1 6.63
CHEMBL1673040 1 6.53
CHEMBL1673048 1 6.47
CHEMBL1673046 1 6.44
CHEMBL5686989 1 6.06
CHEMBL1673057 1 5.95
CHEMBL5687005 1 5.90
CHEMBL1672987 1 5.84
CHEMBL4632948 1 5.82
CHEMBL5687010 1 5.76
CHEMBL5687020 1 5.61
CHEMBL1673056 1 5.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1673051 IC50 = 66.0 nM 7.18
CHEMBL5687018 IC50 = 82.0 nM 7.09
CHEMBL1673039 IC50 = 87.0 nM 7.06
CHEMBL1673052 IC50 = 98.0 nM 7.01
CHEMBL1673043 IC50 = 114.0 nM 6.94
CHEMBL5686992 IC50 = 140.0 nM 6.85
CHEMBL1673053 IC50 = 146.0 nM 6.84
CHEMBL2381340 IC50 = 162.0 nM 6.79
CHEMBL1673047 IC50 = 171.0 nM 6.77
CHEMBL2381339 IC50 = 199.0 nM 6.70
CHEMBL1673041 IC50 = 233.0 nM 6.63
CHEMBL1673040 IC50 = 297.0 nM 6.53
CHEMBL1673048 IC50 = 338.0 nM 6.47
CHEMBL1673046 IC50 = 364.0 nM 6.44
CHEMBL5686989 IC50 = 873.0 nM 6.06
CHEMBL1673057 IC50 = 1130.0 nM 5.95
CHEMBL5687005 IC50 = 1253.0 nM 5.90
CHEMBL1672987 IC50 = 1450.0 nM 5.84
CHEMBL4632948 IC50 = 1530.0 nM 5.82
CHEMBL5687010 IC50 = 1724.0 nM 5.76
CHEMBL5687020 IC50 = 2460.0 nM 5.61
CHEMBL1673056 IC50 = 5090.0 nM 5.29