Assay Detail
Binding
CHEMBL5708937
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at full length human TRPA1 transfected in HEK293F cells assessed as increase in Ca2+ influx by FLIPR calcium assay
7
Total Activities
7
Compounds Tested
1
Activity Types
4
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293F |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL6007) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Species comparison and pharmacological characterization of human, monkey, rat, and mouse TRPA1 channels.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 7 | 4.94 | 5.31 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | HP:0012531 |
| EFO_ID | EFO_ID | - | — | HP:0002099 |
| EFO_TERM | EFO_TERM | - | — | pain |
| EFO_TERM | EFO_TERM | - | — | asthma |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL23293 | SALIRASIB | 2.0 | 1 | 5.31 |
| CHEMBL184238 | URB-597 | 1.0 | 1 | 5.12 |
| CHEMBL454280 | 4-HYDROXYNON-2-ENAL | — | 1 | 5.00 |
| CHEMBL293492 | CINNAMALDEHYDE | -1.0 | 1 | 4.90 |
| CHEMBL482477 | — | — | 1 | 4.39 |
| CHEMBL108541 | TRINITROPHENOL | -1.0 | 1 | - |
| CHEMBL256087 | MENTHOL | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL23293 | SALIRASIB | EC50 | = | 4900.0 | nM | 5.31 |
| CHEMBL184238 | URB-597 | EC50 | = | 7500.0 | nM | 5.12 |
| CHEMBL454280 | 4-HYDROXYNON-2-ENAL | EC50 | = | 9900.0 | nM | 5.00 |
| CHEMBL293492 | CINNAMALDEHYDE | EC50 | = | 12500.0 | nM | 4.90 |
| CHEMBL482477 | — | EC50 | = | 40400.0 | nM | 4.39 |
| CHEMBL108541 | TRINITROPHENOL | EC50 | = | 107000.0 | nM | - |
| CHEMBL256087 | MENTHOL | EC50 | = | 278000.0 | nM | - |