Assay Detail
Binding
CHEMBL5709117
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Inhibition of GST-tagged NUAK1 A195T (unknown origin) expressed in HEK293 cells using ALNRTSSDSALHRRR as substrate incubated for 30 mins in presence of [gamma-32P]-ATP by Cerenkov counting method
4
Total Activities
4
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.83 | 6.22 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_TERM | EFO_TERM | - | — | cancer |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5303523 | — | — | 1 | 6.22 |
| CHEMBL4303523 | — | — | 1 | 6.05 |
| CHEMBL5190521 | — | — | 1 | 5.82 |
| CHEMBL4303250 | — | — | 1 | 5.23 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5303523 | — | IC50 | = | 600.0 | nM | 6.22 |
| CHEMBL4303523 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL5190521 | — | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL4303250 | — | IC50 | = | 5888.0 | nM | 5.23 |