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Assay Detail

CHEMBL5711926

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Binding
Covalent-allosteric inhibition of full length N-terminal his6-tagged AKT (2 to 446 residues) (unknown origin) expressed in Spodoptera frugiperda Sf9 cells by HTRF analysis
16
Total Activities
16
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RAC-alpha serine/threonine-protein kinase (CHEMBL4282)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structural and chemical insights into the covalent-allosteric inhibition of the protein kinase Akt.
Uhlenbrock N, Smith S, Weisner J, Landel I, Lindemann M, Le TA, Hardick J, Gontla R, Scheinpflug R, Czodrowski P, Janning P, Depta L, Quambusch L, Müller MP, Engels B, Rauh D.
Chem Sci (2019) 10 : 3573 -3585
PubMed 30996949 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.00 9.10

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0007254
EFO_TERM EFO_TERM - breast cancer

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4795065 Borussertib 1 9.10
CHEMBL2325741 CAPIVASERTIB 4.0 1 9.05
CHEMBL5712063 1 8.92
CHEMBL5712048 1 8.52
CHEMBL2177390 IPATASERTIB 3.0 1 8.46
CHEMBL5712042 1 8.44
CHEMBL5712046 1 8.43
CHEMBL5712049 1 8.04
CHEMBL1079175 MK-2206 2.0 1 8.00
CHEMBL5712045 1 7.97
CHEMBL5712066 1 7.85
CHEMBL5712061 1 7.74
CHEMBL5712044 1 7.28
CHEMBL5712058 1 6.90
CHEMBL5712059 1 6.72
CHEMBL5712055 1 6.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4795065 Borussertib IC50 = 0.8 nM 9.10
CHEMBL2325741 CAPIVASERTIB IC50 = 0.9 nM 9.05
CHEMBL5712063 IC50 = 1.2 nM 8.92
CHEMBL5712048 IC50 = 3.0 nM 8.52
CHEMBL2177390 IPATASERTIB IC50 = 3.5 nM 8.46
CHEMBL5712042 IC50 = 3.6 nM 8.44
CHEMBL5712046 IC50 = 3.7 nM 8.43
CHEMBL5712049 IC50 = 9.1 nM 8.04
CHEMBL1079175 MK-2206 IC50 = 10.0 nM 8.00
CHEMBL5712045 IC50 = 10.8 nM 7.97
CHEMBL5712066 IC50 = 14.0 nM 7.85
CHEMBL5712061 IC50 = 18.1 nM 7.74
CHEMBL5712044 IC50 = 52.8 nM 7.28
CHEMBL5712058 IC50 = 126.0 nM 6.90
CHEMBL5712059 IC50 = 192.0 nM 6.72
CHEMBL5712055 IC50 = 285.0 nM 6.54