Assay Detail
Binding
CHEMBL5719316
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of AKT in Melphalan Resistant human RPMI-8226 cells assessed as reduction of cell growth incubated for 72 hrs by CellTiter 96 aqueous one solution cell proliferation assay
4
Total Activities
4
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Melphalan-resistant RPMI-8226 cell |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase AKT (CHEMBL2111353) ↗| Type | PROTEIN FAMILY |
| Organism | Homo sapiens |
Publication
Small molecule inhibitor screen identifies synergistic activity of the bromodomain inhibitor CPI203 and bortezomib in drug resistant myeloma.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.59 | 6.56 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | EFO:0001378 |
| EFO_TERM | EFO_TERM | - | — | multiple myeloma |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1186409 | — | — | 1 | 6.56 |
| CHEMBL379218 | — | — | 1 | 5.81 |
| CHEMBL494089 | GSK-690693 | 1.0 | 1 | 5.00 |
| CHEMBL595583 | — | — | 1 | 5.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1186409 | — | IC50 | = | 277.0 | nM | 6.56 |
| CHEMBL379218 | — | IC50 | = | 1549.8 | nM | 5.81 |
| CHEMBL494089 | GSK-690693 | IC50 | = | 10000.0 | nM | 5.00 |
| CHEMBL595583 | — | IC50 | = | 10000.0 | nM | 5.00 |