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Assay Detail

CHEMBL5721347

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 3H-N-Methylspiperone from DRD4 L3.28F mutant in human HEK293T cells membrane assessed as inhibition constant incubated for 2 hrs by radioligand competition assay
18
Total Activities
9
Compounds Tested
1
Activity Types
6
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Subcellular Membrane
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

D<sub>4</sub> dopamine receptor high-resolution structures enable the discovery of selective agonists.
Wang S, Wacker D, Levit A, Che T, Betz RM, McCorvy JD, Venkatakrishnan AJ, Huang XP, Dror RO, Shoichet BK, Roth BL.
Science (2017) 358 : 381 -386
PubMed 29051383 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 6.94 8.15

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0005090
EFO_ID EFO_ID - MONDO:0005180
EFO_ID EFO_ID - HP:0100543
EFO_TERM EFO_TERM - schizophrenia
EFO_TERM EFO_TERM - parkinson disease
EFO_TERM EFO_TERM - cognitive impairment

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL20734 NEMONAPRIDE 2.0 2 8.15
CHEMBL267932 MESPIPERONE 2.0 2 8.02
CHEMBL267014 L-745870 2 7.11
CHEMBL103772 2 6.89
CHEMBL59 DOPAMINE 4.0 2 6.77
CHEMBL444309 2 6.54
CHEMBL81330 2 6.50
CHEMBL45244 PD-168,077 2 6.39
CHEMBL1616116 2 6.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL20734 NEMONAPRIDE Ki = 7.15 nM 8.15
CHEMBL20734 NEMONAPRIDE Ki = 7.079 nM 8.15
CHEMBL267932 MESPIPERONE Ki = 9.55 nM 8.02
CHEMBL267932 MESPIPERONE Ki = 9.94 nM 8.00
CHEMBL267014 L-745870 Ki = 78.1 nM 7.11
CHEMBL267014 L-745870 Ki = 77.62 nM 7.11
CHEMBL103772 Ki = 128.82 nM 6.89
CHEMBL103772 Ki = 128.0 nM 6.89
CHEMBL59 DOPAMINE Ki = 169.82 nM 6.77
CHEMBL59 DOPAMINE Ki = 172.0 nM 6.76
CHEMBL444309 Ki = 288.4 nM 6.54
CHEMBL444309 Ki = 292.0 nM 6.54
CHEMBL81330 Ki = 316.23 nM 6.50
CHEMBL81330 Ki = 326.0 nM 6.49
CHEMBL45244 PD-168,077 Ki = 407.38 nM 6.39
CHEMBL45244 PD-168,077 Ki = 410.0 nM 6.39
CHEMBL1616116 Ki = 794.33 nM 6.10
CHEMBL1616116 Ki = 806.0 nM 6.09