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Assay Detail

CHEMBL5721354

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 3H-N-Methylspiperone from DRD2 F3.28L mutant in human HEK293T cells membrane assessed as inhibition constant incubated for 2 hrs by radioligand competition assay
18
Total Activities
9
Compounds Tested
1
Activity Types
6
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293T
Subcellular Membrane
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

D<sub>4</sub> dopamine receptor high-resolution structures enable the discovery of selective agonists.
Wang S, Wacker D, Levit A, Che T, Betz RM, McCorvy JD, Venkatakrishnan AJ, Huang XP, Dror RO, Shoichet BK, Roth BL.
Science (2017) 358 : 381 -386
PubMed 29051383 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 6.61 9.24

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0005090
EFO_ID EFO_ID - MONDO:0005180
EFO_ID EFO_ID - HP:0100543
EFO_TERM EFO_TERM - schizophrenia
EFO_TERM EFO_TERM - parkinson disease
EFO_TERM EFO_TERM - cognitive impairment

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL267932 MESPIPERONE 2.0 2 9.24
CHEMBL20734 NEMONAPRIDE 2.0 2 9.22
CHEMBL81330 2 6.15
CHEMBL267014 L-745870 2 6.13
CHEMBL444309 2 5.85
CHEMBL45244 PD-168,077 2 5.77
CHEMBL59 DOPAMINE 4.0 2 5.76
CHEMBL1616116 2 5.75
CHEMBL103772 2 5.74

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL267932 MESPIPERONE Ki = 0.58 nM 9.24
CHEMBL267932 MESPIPERONE Ki = 0.5754 nM 9.24
CHEMBL20734 NEMONAPRIDE Ki = 0.6026 nM 9.22
CHEMBL20734 NEMONAPRIDE Ki = 0.61 nM 9.21
CHEMBL81330 Ki = 711.0 nM 6.15
CHEMBL81330 Ki = 707.95 nM 6.15
CHEMBL267014 L-745870 Ki = 741.31 nM 6.13
CHEMBL267014 L-745870 Ki = 751.0 nM 6.12
CHEMBL444309 Ki = 1412.54 nM 5.85
CHEMBL444309 Ki = 1432.0 nM 5.84
CHEMBL45244 PD-168,077 Ki = 1698.24 nM 5.77
CHEMBL59 DOPAMINE Ki = 1737.8 nM 5.76
CHEMBL1616116 Ki = 1778.28 nM 5.75
CHEMBL103772 Ki = 1819.7 nM 5.74
CHEMBL59 DOPAMINE Ki = 1829.0 nM 5.74
CHEMBL103772 Ki = 1866.0 nM 5.73
CHEMBL1616116 Ki = 1863.0 nM 5.73
CHEMBL45244 PD-168,077 Ki = 2108.0 nM 5.68